In the medical field, new medicines, treatment methods and procedures are introduced at such a dazzling pace that even medical experts struggle to stay up to date. In the field of diagnostics many advances have also been made. If a disease can be diagnosed during its early stages it is easier to treat and the chances of success are much better. ELISA kits have proven to be a major breakthrough. The enzyme linked immunosorbent assay enables diagnosticians to recognize certain conditions quickly.
The enzyme linked immunosorbent assay, or EIA as it is known for short is performed in a laboratory. The test is easy to run and many tests can be performed at the same time. The purpose of the test is to measure the concentration of both antigens and antibodies in the blood sample. The ability to measure this concentration is a very important part of the diagnostic process.
When there are antibodies present in a blood sample it means that the body has produced them to counteract the effects of a harmful substance, called antigens. If there are antibodies present, it is certain that there are also antigens because the antibodies are only produced when a threat is detected. By identifying the specific type of antibody produced by the body it is also possible to determine the exact nature of the threat.
Patients experience no pain when the test is administered. A small quantity of blood is taken and then sent to a laboratory where a technician has already prepared a number of petri dishes, each containing a different antigen. The blood sample is then added to the dishes. If the antibodies in the blood samples react to any of the antigens they can be identified.
There are different forms of the EIA test, ranging from very simple to extremely complex. Different kits are used for the different forms of the test. With the direct EIA test, for example, the antigen is first covered with a multiwall plate. In this case the antibody in the blood sample will only react with the antigen if specific enzymes are added.
With the indirect EIA test polystyrene plate is used to cover the antigen. This allows the technician to process the test in two distinct stages before the antibody will be able to identify the antigen and react with it. During each stage it is possible to identify both primary and secondary antibodies. The benefit of this ability is that it saves money and time.
Matched pairs of antibodies are used in the processing of the sandwich EIA test. In this case one of the antibodies is coated with polystyrene while the second one acts as a detecting agent. This test is performed when it is deemed necessary to determine the concentration of analytes in the sample. It is extremely accurate and can even detect diseases that have not manifested yet.
The EIA test is so popular with medical experts because it is versatile, quick and very accurate. The test is easy to administer and to process too. Patients benefit because their conditions can be diagnoses at an earlier stage. This makes treatment easier and it improves the prognosis.
The enzyme linked immunosorbent assay, or EIA as it is known for short is performed in a laboratory. The test is easy to run and many tests can be performed at the same time. The purpose of the test is to measure the concentration of both antigens and antibodies in the blood sample. The ability to measure this concentration is a very important part of the diagnostic process.
When there are antibodies present in a blood sample it means that the body has produced them to counteract the effects of a harmful substance, called antigens. If there are antibodies present, it is certain that there are also antigens because the antibodies are only produced when a threat is detected. By identifying the specific type of antibody produced by the body it is also possible to determine the exact nature of the threat.
Patients experience no pain when the test is administered. A small quantity of blood is taken and then sent to a laboratory where a technician has already prepared a number of petri dishes, each containing a different antigen. The blood sample is then added to the dishes. If the antibodies in the blood samples react to any of the antigens they can be identified.
There are different forms of the EIA test, ranging from very simple to extremely complex. Different kits are used for the different forms of the test. With the direct EIA test, for example, the antigen is first covered with a multiwall plate. In this case the antibody in the blood sample will only react with the antigen if specific enzymes are added.
With the indirect EIA test polystyrene plate is used to cover the antigen. This allows the technician to process the test in two distinct stages before the antibody will be able to identify the antigen and react with it. During each stage it is possible to identify both primary and secondary antibodies. The benefit of this ability is that it saves money and time.
Matched pairs of antibodies are used in the processing of the sandwich EIA test. In this case one of the antibodies is coated with polystyrene while the second one acts as a detecting agent. This test is performed when it is deemed necessary to determine the concentration of analytes in the sample. It is extremely accurate and can even detect diseases that have not manifested yet.
The EIA test is so popular with medical experts because it is versatile, quick and very accurate. The test is easy to administer and to process too. Patients benefit because their conditions can be diagnoses at an earlier stage. This makes treatment easier and it improves the prognosis.
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